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The primary source of human listeriosis is the food we eat. LM is considered one of the most hazardous food-borne pathogens due to its ability to grow at refrigeration temperatures. Therefore, LM is able to survive in a wide variety of food products at ambient temperatures for long periods of time. Listeria monocytogenes bacteria exhibit a number of unique features that make them particularly difficult to kill by conventional methods, including their ability to tolerate a wide range of environmental conditions, including a low pH and a variety of toxic compounds and stressful conditions, such as those encountered in the gut of many food-producing animals. For example, Listeria spp. have the ability to metabolize the amino acid leucine as a carbon source, which enables them to utilize the leucine as an energy source, even when present at a very low pH, at levels of salt, and at temperatures as low as 4° C.
Current detection methods used for Listeria are culture-based, such as detecting Listeria spp. on a Listeria selective agar plate and the subsequent isolation of the bacteria. Many of these conventional methods are time-consuming and labor-intensive. Because of the poor growth characteristics and slow growth rates of Listeria spp.
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